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Species cross reactivity not tested 

2°C to 8°C  (≤ 30 days)

For long-term storage (> 30 days), aliquot and store at ≤ –20°C. Avoid repeated freeze-thaw.

Quidel Polyclonal Antiserum to C4a Protein。   For Research Use Only. Not for use in diagnostic procedures

Highly purified human C4a was isolated and used to immunize rabbits. The Anti-Human C4a polyclonal antisera was tested by immunodiffusion methods and determinded to be monospecific for the C4a protein

Under normal conditions, activation of the classical or lectin complement pathways results in the cleavage of the complement protein, C4 into C4a and C4b by the protease C1s. C4a is rapidly cleaved to its more stable, less active form C4a-des Arg by endogenous serum carboxypeptidase N enzyme. Thus, detection of C4a-des Arg provides a reliable evaluation of classical or lectin complement pathway activation

Applications of Anti-Human C4a antisera have been evaluated, and include immunodiffusion, Western Blot and ELISA