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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
Anti-Mouse IgG2c Rabbit Monoclonal Antibody, Clone RM223
品牌：RevMAb
貨號：31-1103-00
規(guī)格：100ug
貨期：電詢
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Product Details
Product Name Anti-Mouse IgG2c Rabbit Monoclonal Antibody [RM223]
Product Description Rabbit monoclonal to Mouse IgG2c
Catalog No. 31-1103-00
Clone Name RM223
Product Data Sheet Anti-Mouse IgG2c Rabbit Monoclonal Antibody Data Sheet RM223
Specificity RM223 reacts to the Fc region of mouse IgG2c. No cross reactivity with mouse IgG1, IgG2a, IgG2b, IgG3, IgM, IgA, IgE, human IgG, or rat IgG.
Immunogen Mouse IgG2c
Application Western Blot (WB) (nonreduced only)
Immunoprecipitation (IP)
Immunohistochemistry (IHC)
Flow Cytometry (FC)
Immunocytochemistry (ICC)
ELISA
Species Reactivity Mouse
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage ELISA: 0.01 ug/mL – 0.2 ug/mL
Immunocytochemistry (ICC): 0.5 ug/mL-2 ug/mL
Immunohistochemistry (IHC): 0.5 ug/mL-2 ug/mL
Western Blot (WB): 0.5 ug/mL-2 ug/mL
Keywords
.
Product Images
ELISA

ELISA of IgG from BalB/C and C57BL6 shows RM223
 reacts to C57BL6 IgG containing IgG2c, and does not
react to BalB/C IgG containing IgG2a. 200 ng/mL, 50
ng/mL, or 10 ng/mL of RM223 was used as the primary
antibody. An alkaline phosphatase conjugated anti-rabbit
IgG as the secondary antibody.

A

A titer ELISA of mouse IgG2c. The plate was
coated with different amounts of mouse IgG2c. A
 serial dilution of RM223 was used as the primary
antibody. An alkaline phosphatase conjugated
anti-rabbit IgG as the secondary antibody.

Western

Western blot of nonreduced(-) and reduced(+)
mouse IgG2c, using 0.5ug/mL of RevMAb clone
RM223. This antibody reacts to nonreduced
IgG2c


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