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Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody, Clone RM135
品牌：RevMAb
貨號(hào)：31-1037-00
規(guī)格：100 ug
貨期：電詢(xún)
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Product Details
Product Name Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody [RM135]
Product Description Rabbit monoclonal to Dimethylated Histone H3 Lysine 4, H3K4me2, Histone H3 (dimethyl K4)
Catalog No. 31-1037-00
Clone Name RM135
Product Data Sheet Anti-Dimethyl-Histone H3 (Lys4) Rabbit Monoclonal Antibody Data Sheet RM135
Specificity RM135 reacts to Histone H3 dimethylated at Lysine 4 (K4me2). No cross reactivity with monomethylated Lysine 4 (K4me1), trimethylated Lysine 4 (K4me3), or other methylations in histone H3.
Immunogen A dimethyl-peptide corresponding to Dimethyl-Histone H3 (Lys4).
Application Western Blot (WB)
Immunocytochemistry (ICC)
ELISA
Multiplex
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 0.02 ug/mL – 0.2 ug/mL;
ICC: 1 ug/mL - 2 ug/mL;
ELISA: 0.2 ug/mL - 1 ug/mL;
Multiplex: 0.1 ug/mL – 0.5 ug/mL.
Keywords
.
Product Images
RM135

RM135 specifically reacts to Histone H3 dimethylated
 at Lysine 4 (K4me2). No cross reactivity with mono-
 methylated Lysine 4 (K4me1), trimethylated Lysine 4
(K4me3), or other methylations in histone H3.

Western

Western Blot of recombinant histone H3.3 (1) and
acid extracts of HeLa cells (2), using RM135 at 0.025
ug/mL, showed a band of histone H3 dimethylated
at Lysine 4 (K4me2) in HeLa cells.

Immunocytochemistry

Immunocytochemistry of HeLa cells treated with
sodium butyrate, using Dimethyl-Histone H3 (Lys4)
 Rabbit mAb RM135 (red). Actin filaments have
been labeled with fluorescein phalloidin (green).


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