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Tel:400-968-7988    021-33779008
Anti-Histone H3 (Unmodifed Lys4) Rabbit Monoclonal Antibody, Clone RM186
品牌：RevMAb
貨號：31-1086-00
規(guī)格：100 ug
貨期：電詢
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Product Details
Product Name Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody [RM186]
Product Description Rabbit Monoclonal to Histone H3 (Unmodified Lys4); Histone H3 (Unmodified Lysine 4)
Catalog No. 31-1086-00
Clone Name RM186
Product Data Sheet Anti-Histone H3 (Unmodified Lys4) Rabbit Monoclonal Antibody Data Sheet RM186
Specificity RM186 recognizes Histone H3 unmodified at Lys4 and does not recognize acetylated or methylated Lys4. The antibody binding specificity allows for modifications of Arg2, Thr3, and/or other modifications in Histone H3.
Immunogen An unmodified peptide corresponding to the N-terminus of Histone H3.
Application Western Blot (WB)
ELISA
Multiplex
Immunocytochemistry (ICC)
Species Reactivity Human
Vertebrates
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Size 100 ug
Concentration 1.0 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Western Blot: 1 ug/mL – 2 ug/mL;
ELISA: 0.5 ug/mL - 1 ug/mL;
Multiplex: 0.5 ug/mL – 2 ug/mL;
Immunocytochemistry: 0.5 ug/mL- 2 ug/mL.
Keywords
.
Product Images
RM186

RM186 specifically recognizes Histone H3 unmodified
 at Lys4 and does not recognize acetylated, mono-
 methylated, dimethylated, or trimethylated Lys4. The
 antibody binding specificity allows for modifications of
 Arg2 or Thr3 in histone H3.

Western

Western Blot of acid extracts of HeLa cells treated with
sodium butyrate (HeLa+NaBu), treated with Nocodazole
(HeLa+Noc), or non-treated; and recombinant histone
 H3.1 protein, using RM186 at 1 ug/mL, showed a band
 of histone H3 with unmodified Lys4.

Immunocytochemical

Immunocytochemical staining of HeLa cells treated
 with sodium butyrate, using anti-Acetyl-Histone
H3 (Unmodified Lys4) Rabbit Monoclonal Antibody (clone
RM186) (red). Actin filaments have been labeled
with fluorescein phalloidin (green).


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