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上海拜力生物科技公司
Tel:400-968-7988    021-33779008
Anti-BRAF V600E Rabbit Monoclonal Antibody, Clone RM8; BRAF(V600E)
品牌：RevMAb
貨號(hào)：31-1042-00
規(guī)格：100ug
貨期：電詢
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Product Details
Product Name BRAF V600E Rabbit Monoclonal Antibody [RM8]
Product Description Rabbit monoclonal to BRAF V600E Mutant, BRAF(V600E), BRAF-V600E
Catalog No. 31-1042-00
Clone Name RM8
Product Data Sheet BRAF V600E Rabbit Monoclonal Antibody Data Sheet
Specificity RM8 reacts to the BRAF V600E mutant. No cross reactivity with wild type BRAF.
Immunogen A peptide corresponding to BRAF V600E mutant
Application Immunohistochemistry
Immunocytochemistry
Western Blot
Species Reactivity Human
Conjugate None
Intended Use Research Use Only
.
Properties
Form Liquid
Storage Instructions Store at -20.0°C
Stability Stable for 1 Year at -20.0°C from date of receipt
Storage Buffer 50% Glycerol/PBS with 1% BSA and 0.09% sodium azide
Volume/Item Size 100ug
Concentration 1 mg/mL
Purity Protein A affinity purified from an animal origin–free culture supernatant
Clone Type Monoclonal
Isotype Rabbit IgG
Usage Immunohistochemistry (IHC): 0.5 – 5 ug/mL;
Immunocytochemistry (ICC): 2 – 10 ug/mL;
Western Blot (WB): 0.5 – 2 ug/mL
References for BRAF V600E Mutant antibody [RM8] Sun HL et al. ERK Activation Globally Downregulates miRNAs through Phosphorylating Exportin-5. Cancer Cell 10.1016/j.ccell.2016.10.001. WB; Human. Read more (PubMed: 27846390)

Yang Het al. Activated MEK cooperates with Cdkn2a and Pten loss to promote the development and maintenance of melanoma. Oncogene 10.1038/onc.2016.526. WB; Human. Read more (PubMed: 28263969)
Protocols BRAF V600E IHC Protocol
Product Images
Immunohistochemical

Immunohistochemical staining of formalin fixed and
 paraffin embedded melanoma tissue sections.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded Thyroid Carcinoma tissue. Allele
 specific PCR validated to be Negative for BRAF V600E.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
paraffin embedded Thyroid Carcinoma tissue. Allele
 specific PCR validated to be Positive for BRAF V600E.

Western

Western Blot analysis of cell lysates prepared from cell
lines expressing endogenous mutant (V600E) BRAF or
wild type (WT) protein.

Immunohistochemical

Immunohistochemical staining of formalin fixed and
 paraffin embedded human cell line sections.


WiDr Cells (400X, Positive)

Immunohistochemical

Immunohistochemical staining of formalin fixed and
 paraffin embedded human cell line sections.



SW40 Cells (400X, Negative)


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