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IL-10 is an immunosuppressive cytokine produced by a variety of mammalian cell types including macrophages, monocytes, T cells, B cells and keratinocytes.? IL-10 inhibits the expression of proinflammatory cytokines such as IL-1 and TNF-α.? Like IL-4, IL-10 enhances humoral immune responses and attenuates cell-mediated immune reactions.? Human IL-10 is active on murine cells, but murine IL-10 is inactive on human cells.? Recombinant Human IL-10 is an 18.6 kDa protein of 161 amino acid residues.

Source: E.coli

Synonyms: B-TCGF, CSIF, TGIF

AA Sequence: MSPGQGTQSE NSCTHFPGNL PNMLRDLRDA FSRVKTFFQM KDQLDNLLLK ESLLEDFKGY LGCQALSEMI QFYLEEVMPQ AENQDPDIKA HVNSLGENLK TLRLRLRRCH RFLPCENKSK AVEQVKNAFN KLQEKGIYKA MSEFDIFINY IEAYMTMKIR N

Purity: ≥ 98% by SDS-PAGE gel and HPLC analyses.

Biological Activity: Determined by the dose-dependent co-stimulation (with human IL-4) of MC/9 cells. The ED₅₀ was found to be ≤ 2.0 ng/ml, corresponding to a specific activity of ≥ 5 x 10⁵ units/mg.