The new Badrilla SiteCounter^TM S-Palmitoylated protein kit is a quick and easy way to quantify the extent of S-palmitoylation of your protein of interest. Using a straightforward mass tag approach, modification of each cysteine residue results in an incremental increase in the mass of the protein.  The number of mobility shifts (as detected by western blot analysis) is observed as a ladder of protein bands, each ‘step’ in the ladder representing an additional S-palmitoylated cysteine residue. 

Kit highlights

• Enables determination of the number of S-palmitoylation sites on a protein
• Enables calculation of S-palmitoylation stoichiometry
• Kit designed to process 2 x 6 biological samples with paired negative controls (i.e. 24 samples in total).
• Convenient stopping point after blocking treatment.
• Assay suitable for downstream western blot analysis

References

1. Percher A., et al. (2016) Mass-tag labelling reveals site specific and endogenous levels of protein S-fatty acylation. PNAS 113: 4302–4307
2. Yokoi N., et al. (2016) Identification of PSD-95 Depalmitoylating Enzymes. J. Neurosc., 36:6431– 6444