A fully validated, highly specific, affinity purified mouse monoclonal antibody to phospholamban (PLB, PLN)

The majority of calcium required for muscle contraction is mobilised from the sarcoplasmic reticulum (SR), to which it has to return to in order to facilitate relaxation. The (Ca²⁺- Mg²⁺) ATPase enzyme is responsible for the sequestration of calcium after its release from the SR, and is controlled by phospholamban (PLB) in cardiac, smooth and slow-twitch skeletal muscle.

The monoclonal antibody (A1) was raised against bovine cardiac PLB  and recognises an epitope comprising of residues ⁷LTRSAIRRAS¹⁶. The antibody reacts with all forms of phospholamban PLB: monomer, oligomers, phosphorylated and non-phosphorylated.

The antibody stimulates Ca²⁺ pump activity upon binding to PLB, to an extent equivalent to stoichiometric phosphorylation of PLB Manipulation of SERCA2:PLB ratios is a therapeutic strategy for heart failure. Elimination of PLB boosts SR Ca²⁺-load which can exacerbate pathological outcomes (apoptosis, arrhythmias) when combined with other alterations in SR function.

Tested applications: Western blot (1:5000 dilution), IHC Microscopy (1:200 dilution), Ca²⁺ pump assays (1:500 dilution).