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In previous studies, dual SMAD inhibition using small molecule inhibitors of BMP and TGFβ pathways, i.e., LDN193189 and SB431542 respectively, has been shown to induce efficient neural differentiation of human pluripotent stem cells. In a recent publication, it was shown that combination of LDN193189/SB431542 with a specific GSK-3β inhibitor (CHIR99021) could significantly promote the conversion of postnatal human fibroblasts transduced with Ascl1 and Ngn2 into functional neuron-like cells with yields up to 200% and neuronal purities up to 80%.

How to Use:

• In vitro: Dilute received Ready-to-use iN-LSC stock solution at 1:1000 into your neuronal reprograming medium to make the neuron induction media and use it to initiate neuronal reprogramming.

Reference:

1. 1.? Ladewig J, et al. Small molecules enable highly efficient neuronal conversion of human fibroblasts. (2012) Nature Methods 9(6), p575-578.

Products are for research use only. Not for human use.