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FPH1 is a potent and selective small molecule that induced?Functional?Proliferation of primary human?Hepatocytes in vitro, identified by a high-throughput, cell-based screening using primary human hepatocytes. It induced a significant increase in hepatocyte number and elevated the number of hepatocytes undergoing mitosis in a dose-dependent manner. Cells treated with FPH1 also maintained their liver-specific functions. Immunofluorescence staining for Ki67 and albumin, along with FACS-based cell counting, revealed that hepatocytes from a wide range of genetically diverse individuals could be expanded and normal hepatocytes functions could be maintained after expansion. FPH1 can also promote the differentiation of iPS cells toward a hepatic lineage and promote the maturation of induced hepatic cells toward a more *****-like liver phenotype.

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How to Use:?

• In vitro:?FPH1 was suggested to be supplemented into the culture medium on day 1 and 5 at a concentration of 15-20 μM to? induce functional proliferation of primary human hepatocytes in vitro.
• In vivo:?n/a

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Reference:

• 1. Shan J, et al. Identification of small molecules for human hepatocyte expansion and iPS differentiation. (2013) Nat Chem Biol. 9(8):514-20.?

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