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TAME,UbiquitinLigaseAPC/CInhibitor
品牌：Xcessbio
貨號(hào)：M60246-2s
規(guī)格：2 mg solid
貨期：
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TAME,UbiquitinLigaseAPC/CInhibitor
商品詳情 參考文獻(xiàn) 相關(guān)資料
Product Information
Molecular Weight: 378.87
Formula: C14H22N4O4S.HCl
Purity: ≥98%
CAS#: 1784-03-8
Solubility: DMSO and water up to 100 mM
Chemical Name: (S)-methyl 5-guanidino-2-(4-methylphenylsulfonamido)pentanoate
Storage: Powder: 4oC 1 year. DMSO: 4oC 3 month; -20oC 1 year.

Biological Activity:

TAME is a competitive inhibitor of the ubiquitin ligase anaphase-promoting complex/cyclosome (APC/C). It promotes Cdc20 autoubiquitination and inhibits cyclin proteolysis in mitotic Xenopus oocyte extracts (IC50 ~ 12 μM) and blocks cyclin degradation in interphase extract.

How to Use:

In vitro: TAME was used at 50-200 μM final concentration in various in vitro assays.
In vivo: n/a

Reference:

  • 1. Zeng X, et al. Pharmacologic inhibition of the anaphase-promoting complex induces a spindle checkpoint-dependent mitotic arrest in the absence of spindle damage. (2010) Cancer Cell. 18(4):382-95.?
  • 2. Zeng X, et al. An APC/C inhibitor stabilizes cyclin B1 by prematurely terminating ubiquitination. (2012) Nat Chem Biol. 8(4):383-92.?


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