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Anti-Complement component C9 (human) ABS 004-22

品牌：

貨號：ABS 004-22

規(guī)格：200 μg

貨期：

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Anti-Complement component C9 (human)

商品詳情參考文獻相關(guān)資料

Cat. No.: ABS 004-22
Product name: Anti-Complement component C9 (human)
Description: Mouse monoclonal antibody?
Specificity: ABS 004-22 is specific for complement factor C9
Immunogen: Complement factor C9
Application: ELISA, WB, IHC, IF
Species reactivity (positive): Human
Species reactivity (negative): Not determined
Subclass: IgG1/k
Gene ID: 735
Clone number: 22
Epitope: Not determined
Content: Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.
Purification: Protein-A purified
Form: Liquid
Solvent: 0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage: 4-8oC without exposure to light. No precautions necessary during handling.
Target: Complement factor C9 is a 71kDa single-chain ellipsoid a-globular molecule of 558 amino acids including a leader sequence of 20 amino acids. C9 bind the C5b8 complex on membranes and has an unique property in forming tubular structures containing between 12 and 18 monomers, closely resembling the membrane attack complex (MAC). The primary site of synthesis is hepatocytes, secondary sites are monocytes, fibroblasts and glial cells. The normal level of C9 in plasma is 60 μg/mL, and the level is up regulated from hepatocytes by IFN-g.?

Application 1: ELISA

ABS 004-22 recognizes C9 in human serum diluted 1/50 in Tris buffer (20 mM Tris-base, 1 mM MgCl2, 1 mM CaCl2 and 140 mM NaCl) and incubated for 2 hours at 37°C using a human IgM coated (10 μg/mL overnight at 4°C, blocked with PBS 7.2 + 1% BSA for 1 hour) ELISA plate.
Application 2: WB
ABS 004-22 can be used in Western blotting.
Application 3: IHC

ABS 004-22 can be used in IHC.

IHC staining of human liver with ABS 004-22. Plasma of portal veins are strongly stained. Dilution: 1:100.

IHC staining of tonsil tissue with ABS 004-22. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Also moderate staining were observed from plasma of veins. Dilution: 1:100.
Application 4: IF

ABS 004-22 can be used in IF.

IF staining of tonsil tissue with ABS 004-22. Follicular dendritic cell network stained strongly in germinal centers of tonsil tissue. Dilution: 1:75.

IF staining of human kidney with ABS 004-22. Plasma of veins are strongly stained. Dilution: 1:75.