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Anti-Procollagen type IIA N-terminal propeptide (human, PIIANP) BTE 003-02
品牌：
貨號：BTE 003-02
規(guī)格：200 μg
貨期：
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Anti-Procollagen type IIA N-terminal propeptide (human, PIIANP)
商品詳情 參考文獻(xiàn) 相關(guān)資料
Cat. No.
BTE 003-02
Product name
Anti-Procollagen type IIA N-terminal propeptide (human, PIIANP)
Description

Mouse monoclonal antibody

Specificity

BTE 003-02 is specific for human PIIANP

Immunogen

Recombinant expressed PIIANP

Application
ELISA, IHC-P
Species reactivity (positive)
Human
Species reactivity (negative)
Not determined
Subclass

IgG1/k

Gene ID
1277
Clone number
NA
Epitope

Epitope is located at the amino-terminal part of procollagen type IIA

Content

Available in 200 μL and 1 mL size.1 mg/mL +/- 15%. See Certificate of Analysis for details.

Purification
Protein-A purified
Form
Liquid
Solvent
0.01 M phosphate buffer, pH 7.4, containing 0.5 M NaCl and 15 mM sodium azide
Storage
4-8oC without exposure to light. No precautions necessary during handling.
Target

Collagen type II is the most abundant protein of cartilage matrix. Two isoforms exist and collagen type IIA is involved in early development of cartilage in embryos. The collagen is produced from type II procollagen when two teloterminal propeptides are digested from the procursor and released into the circulation. Procollagen type II N-terminal propeptide (PIIANP) is considered a marker of cartilage synthesis and the normal concentration in serum is approximately 30ng/mL.

Application 1

ELISA
BTE 003-02 also reacts with recombinant PIIANP coated directly onto the microtiter well

Application 2
IHC
BTE 003-02 diluted in 10%FCS/TBS is well suited for immunohistochemistry on formalin-fixed, paraffin-embedded fetal tissue.
Immunohistochemical staining of a formalin-fixed, paraffin-embedded fetal bone anlage in the hand (around week 10) with BTE 003-02.
Immunohistochemical staining of a formalin-fixed, paraffin-embedded biopsy from a chondroblastoma, showing specific staining of tumor cells with AntibodyShop product BTE 003-02 developed with peroxidase-labeled goat anti-mouse Ig (blue color).
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