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Concentration
5u/μl 
5'…CC↓TNAGC…3'
3'…GGANT↑CG…5'

Reaction Conditions 
1X Buffer V4 
10mM Tris-HCl (pH 8.5 at 30°C), 10mM MgCl₂, 100mM KCl, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 100mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol and 50% glycerol. Store at -20°C.

Thermal Inactivation
80°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with Bpu10I, 80% of the DNA fragments can be ligated in the presence of 10% PEG and of these 90% can be recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 2u of Bpu10I for 16 hours at 37°C. 
Supplied with 10X Buffer V4, 10X Buffer UB and Viva Buffer A. (Diluent)
*High enzyme concentration may result in Star Activity.

Ordering Information

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Manual
Bpu10I