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Concentration
1-10u/μl 
5'…AGT↓ACT…3'
3'…TCA↑TGA…5'

Reaction Conditions 
0.5X Buffer UB 
12.5mM Tris-acetate (pH 7.6 at 30°C), 5mM Mg-acetate, 50mM K-acetate, 3.5mM 2-mercaptoethanol, and 25μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 10-fold overdigestion with BmcAI, 70% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 10u of BmcAI for 16 hours at 37°C.
Supplied with 10X Buffer UB and Viva Buffer A. (Diluent)

Ordering Information

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Manual
BmcAI {ScaI}