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Concentration
3-10u/μl
5'…CACNNN↓GTG…3'
3'…GTG↑NNNCAC…5'

Reaction Conditions 
1X Buffer DraIII
10mM Tris-HCl (pH 7.6), 10mM MgCl₂, 200mM KCl, 1mM DTT and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 200mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 100μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
65°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with DraIII, 70% of the DNA fragments can be ligated and recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of DraIII for 16 hours at 37°C.
Supplied with 5X Buffer DraIII, 10X Buffer UB and Viva Buffer A 
*High enzyme concentration may result in Star Activity.

Ordering Information

Downloads
Manual
DraIII