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Concentration
2-5u/μl 
5'…CCTGCA↓GG…3'
3'…GG↑ACGTCC…5'

Reaction Conditions 
1X Buffer V5
30mM Tris-HCl (pH 7.9 at 30°C), 10mM Mg-acetate, 60mM K-acetate, and 100μg/ml BSA. Incubate at 37°C.

Storage Buffer
10mM Tris-HCl (pH 7.5), 50mM KCl, 0.1mM EDTA, 7mM 2-mercaptoethanol, 200μg/ml BSA and 50% glycerol. Store at -20°C.

Thermal Inactivation
80°C for 20 minutes.

Ligation / Recutting Assay 
After 5-fold overdigestion with SbfI, more than 90% of the DNA fragments can be ligated and of these 90% can be recut.

Overdigestion Assay 
An unaltered banding pattern was observed after 1μg of DNA was digested with 5u of SbfI for 16 hours at 37°C.
Supplied with 10X Buffer V5, 10X Buffer UB and Viva Buffer A. (Diluent)
* High enzyme concentration may result in Star Activity.

Ordering Information

Downloads
Manual
SbfI