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Description
ViPrimePLUS One Step AtTaq RT-qPCR Master Mix is next generation first choice mix designed for fast and easy one step real-time PCR reaction set up. The improved formulation of master mix contains unique thermostable M-MULV enzyme, Hot Start Taq DNA Polymerases as well as MgCl2 and buffer components at optimal concentrations. The M-MULV enzyme has an optimal operating temperature of 55°C, and has a higher affinity for primer template duplexes which allows very rapid processing during RT step. The One Step AtTaq RT-qPCR Master Mix is designed to achieve excellent results in reaction efficiency, correlation coefficient and slope.
ViPrimePLUS One Step AtTaq RT-qPCR Master Mix can be used to amplify any RNA template including mRNA, total RNA and viral sequences. The improved formulation of RT-qPCR master mix can detect extremely low copy number targets very specifically with high efficiency that gives CT values close to the theoretical time of detection. The ViPrimePLUS One Step AtTaq RT-qPCR Master Mix is a complete system for use in one step real-time PCR, the removal of a separate reverse transcription step reduces handling errors as well as the time taken to obtain results. The formation of primer dimers and non-specific products is prevented leading to optimum sensitivity and specificity..
ViPrimePLUS One Step AtTaq RT-qPCR Master Mix has several formulations optimized to be used with most of real-time PCR instruments. The improved sensitivity and consistency of ViPrimePLUS One Step AtTaq RT-qPCR Master Mix in standard cycling conditions gives the industry leading performance in fast cycling conditions.

Applications
All kinds of RNA sample material suited for RT-qPCR amplification can be used.

Features

• One step real time RT-qPCR reaction set up
• Equipped with thermostable M-MULV enzyme and Hot Start Taq DNA Polymerases
• Reliable quantification of extremely low copy number targets
• Optimal performance for highly sensitive and specific RT-qPCR reaction
• Compatible with most of the real-time PCR platforms

Component
3 x 0.6ml aliquots of master mix 0.6ml aliquots of “no RT control master mix standard”

Storage
Stable at -20°C up to the expiry date stated. Store all components at -20°C upon arrival. Keep in aliquot to reduce freeze-thaw cycles.

Quality Control
As part of the ISO9001:2008 quality assurance systems, each lot of ViPrimePLUS One Step AtTaq RT-qPCR Master Mix has been tested against predetermined specifications to ensure consistent product quality and highest levels of performance and reliability.

Limitation Of Use
For research use only. Not recommended or intended for diagnosis of disease in humans or animals. Do not use internally or externally in humans or animals.

Instruments
To calibrate a real-time PCR reaction, various formulations of master mixes are available for most of the platforms.

Ordering Information

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Manual

ViPrimePLUS One Step AtTaq RT-qPCR MasterMix 
ViPrimePLUS One Step AtTaq RT-qPCR MasterMix with Low ROX 
ViPrimePLUS One Step AtTaq RT-qPCR MasterMix with ROX

Publication
This Product Has Been Used In: 
Attaran, B., Falsafi, T. (2017). Effect of biofilm formation by clinical isolates of Helicobacter pylori on the efflux-mediated resistance to commonly used antibiotics, . World Journal of Gastroenterology, Vol. 23, No. 7. 1163-1170 (2017).