Grade
Ultra Pure Grade
Description
DNase I is a recombinant form of DNase I which is RNase-free, originally isolated from bovine pancreas, is a recombinant enzyme expressed in Pichia pastoris. It is a DNA-specific endonuclease that hydrolyzes the phosphodiester linkages of double- and single-stranded DNA to a mixture of mono- and oligonucleotides.
DNase I manufactured using state-of-the-art processes yielding animal-free material. The enzyme is highly purified and rigorously tested for contaminating RNase and protease activity of RT-PCR. It is an important tool for all applications requiring DNA-free RNA templates and achieving reliable results with undegraded and stable RNA.
Specification
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Appearance
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Colorless to light yellow, clear solution.
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Source
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Bovine pancreas.
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Unit Definition
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One unit according to Kunitz produces an increase in absorbance of 0.001/minute under assay conditions in 1ml at 260nm.
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Storage
Store at -20°C
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Assay
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Resuls
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Volume act. with calf thymys DNA (Kunitz)
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11875 u/ml
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Volume act. with calf thymys DNA (Kunitz)
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993 KU/ml
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Protease up to 50U w.casein resorufinm
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Not detectable.
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RNases up to 10U with MS II RNA
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Not detectable.
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Ordering Information
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Catalog No
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Description
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Pack Size
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PC0719 - 50kU
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Deoxyribonuclease I (DNase I) - For RNA work
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50kU
|
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PC0719 - 100kU
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Deoxyribonuclease I (DNase I) - For RNA work
|
100kU
|
Download
MSDS
Deoxyribonuclease I (DNase I) - For RNA work
