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Description
This lambda marker is suitable for sizing linear double-stranded DNA fragments in agarose and polyacrylamide gels. The lambda DNA is completely digested with PstI and yields 29 fragments in size (in bp): *11501, 5077, 4749, 4507, *2556, 2459, 2443, 2140, 1986, 1700, 1159, 1093, 805, 514, 468, 448, 339, 264 and 247. 216, 211, 200, 164, 150, 94, 87, 72, 15 (obscure bands).

Storage Buffer
10mM Tris-HCl (pH 8.0) and 1mM EDTA. Store at -20°C

6X Loading Dye Solution
10mM Tris-HCl (pH 8.0), 60mM EDTA, 0.03% bromophenol blue, 0.03% xylene cyanol FF and 60% glycerol

Usage Recommendation 
Use 0.05-0.10μg of the DNA ladder per 1mm width of gel lane

Ordering Information

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VC Lambda / Pst I Marker